Fig. 6. RGD-to-RGE change in fibulin-5 modifies behavior of MD-MB-231 cells. A. Western-blot showing the expression of the EMT markers E-cadherin, N-cadherin and vimentin in control MDA-MB-231 cells (C), MDA-MB-231 cells tranfected with the full-length cDNA for fibulin-5 (Fibulin-5WT), or transfected with the cDNA carrying the mutation to produce fibulin-5 containing the D56E change within its RGD motif (Fibulin-5RGE). Molecular weight markers are indicated on the right and β-actin was employed as loading control. B. Top, representative images showing attachment of the indicated MDA-MB-231 cells to the ECM components fibronectin, laminin and type IV collagen. Adhesion to standard culture plates was also examined. Bar, 10 µm. Bottom, graphical representation of the number of attached cells. C. Ability of the MDA-MB-231 cells used in this work to bind to the α_v β ₃ integrin was also examined. Top, representative images of the indicated MDA-MB-231 cells attached to the α_v β ₃ integrin. Bar, 10 µm. Bottom, graphical representation of the number of attached cells as indicated.